Evaluation and Modifications

There are mistakes make by different group during the lab that lead to the diverse result. The first human error identified is the broth. When the broth is mixed, it was not mixed equally. Some part have more and some have less, so the group with the more evenly distributed broth will probably have a higher transformational rate. Another human mistake can be cause by the amount of DNA plasmid. If the equipment is not used properly, the group will have less plasmid DNA for the E.coli to transform. Other that have transformed bacteria on other plates is due human error of cross contamination. Either they use the same loop for all the plates or it is because they misplace their lid somewhere and then put it back on the plate.
This lab will be better if the students are able to do the pre-lab preparation themselves. Also, it can be a self experiment after knowing all the background information that is necessary for the lab instead of a follow the instruction lab, it will be more interesting.